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gxstrands

DNA single strands

DNA single strands

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€50.000,00
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Mass
Topology

Linear ssDNA: Our linear ssDNA is fully customized, containing only the specified sequence, with no exogenous sequences other than an AG at the 5' end.

Circular ssDNA: For small-scale production (< 1 mg), we use a minimal backbone of ~300 nt. For large-scale production (> 1 mg), a larger backbone of ~4100 nt is required.
Please note that the 5' end of our sequence always begins with AG.
Sequence should be between 150 to 10,000 nucleotides.

After submitting your order, our team will review your details and get back to you within two working days. We empower your projects by supplying large quantities of single-stranded DNA. 

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Timeline & production process
Our process, from initial inquiry to final delivery, spans approximately 6 weeks.
Customer inquiry
Customer inquiry
Plasmid design
2 days
Plasmid design
Plasmid production
10 to +30 days - depending on sequence
Plasmid production
Intermediate quality control
1 day
Intermediate quality control
Large scale ssDNA production
Up to 14 days - depending on scale
Large scale ssDNA production
Purification & quality control
5 days
Purification & quality control
Shipment
3 days within Europe, 5 days worldwide
Shipment

FAQ's

FAQ's general

What is single-stranded DNA (ssDNA)?

Single-stranded DNA (ssDNA) plays a crucial role in many biological processes like DNA replication and DNA repair. In our cells it usually occurs only transiently when genomic DNA double-helices are separated into the two constituent strands. Multiple families of viruses have single-stranded DNA genomes, for example adeno-associated viruses (AAVs) which are popular gene therapy vectors.

How do double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) differ?

Double-stranded DNA (dsDNA) is composed of two complementary strands that form a helix, bonded together by hydrogen bonds. In contrast, single-stranded DNA (ssDNA) lacks this double helical structure and is mechanically more flexible than dsDNA.

How does gxstrands make single-stranded DNA at scale?

Commonly, single-stranded DNA production is carried out in vitro by chemical synthesis, rolling circle amplification, or other enzymatic processes. At gxstrands, we use a fermentation-based approach, allowing for the production of scalable amounts of DNA single strands with arbitrary sequence and length.

How is gxstrands' single-stranded DNA delivered?

gxstrands' single-stranded DNA is linearized by default. Your single-stranded DNA is delivered freeze-dried at room temperature. Other delivery options, such as delivery in a specific buffer or at a specific concentration, are possible upon request.

How should I resuspend my single-stranded DNA?

1. Spin your tube upon arrival for 5 seconds in a microcentrifuge, to guarantee that your single stranded DNA is at the bottom of the tube.
2. Add water according to the CoA to achieve a concentration of 100 µM.
3. Mix briefly by flicking or vortexing the tube.
4. Incubate overnight at 4 °C or at room temperature for 1 h.
5. Vortex and centrifuge briefly.
6. Verify the final concentration with a nano volume spectrophotometer.

Are there any storage and handling guidelines for single-stranded DNA?

The stability of single stranded DNA is dependent on the buffer as well as the storage temperature. We recommend storage at -20 °C for long term storage and 4 °C for short term storage. Resuspend in nuclease free water or TE-buffer for best results. We also recommend to check our resuspension-protocol for best results.

What is the delivery time of my sequence?

The average delivery time is 6 weeks. However, the exact delivery time varies depending on the complexity of your sequence. Once the template sequence has been successfully synthesized and cloned into a plasmid, we provide you with an updated timeline. For precise information regarding your specific delivery schedule, please contact our customer support team at support@gxstrands.com.

What lengths of synthetic DNA does gxstrands currently offer for synthesis?

We offer single-stranded DNA sequences up to 10,000 nucleotides.

FAQ's ssDNA for gene editing

What are the advantages of ssDNA over dsDNA in gene editing?

  • Reduced toxicity
  • Lower off-target effects
  • Higher target integration
  • High specificity & efficient knock-in templates for homology-directed repair

How does ssDNA complement gene editing tools like CRISPR/Cas?

For precision gene editing tasks such as site-specific insertion of long DNA sequences, our ssDNA can be used as donor templates designed to work in tandem with CRISPR/Cas or other gene editing tools, facilitating the insertion of synthetic genes.

Can gxstrands modify my ssDNA sequence for special gene editing purposes?

Our single-stranded DNA is always built from natural DNA bases, and our fermentation-based process does not introduce special DNA modifications.

FAQ's ssDNA for DNA origami

What types of ssDNA do you supply for DNA origami projects?

We offer high-quality single-stranded DNA molecules in large quantities to accommodate diverse research and production requirements. Our offerings encompass for example scaffold strands with customizable sequences and lengths of up to 10,000 nucleotides.

How do I determine the correct ssDNA sequence and length for my DNA origami project?

The sequence and length depend on your specific DNA origami design. We recommend consulting our design guidelines or contacting our expert team for assistance in choosing the optimal sequence for your project.

What is the turnaround time for custom ssDNA orders for DNA origami?

Turnaround times vary based on the complexity and length of the sequence. Please contact us with your specific requirements for an estimated delivery timeline.

Do you also offer folding of DNA structures?

We supply all the essential DNA strands required for the assembly of DNA origami structures including the scaffold strand and all needed staple oligonucleotides. Our services do not extend to the design, manufacturing or purification of DNA origami structures. Our focus is on delivering high-quality DNA strands to support your specific research and production needs.