Single-stranded DNA

Why Single-Stranded DNA?

Single-Stranded DNA is a nucleic acid without complementary base pairing, found in certain viruses like Adeno-associated Viruses (AAVs) or as an intermediate in DNA replication. The therapeutic potential of single-stranded DNA has gained recognition recently.

Sequence flexibility

Single-stranded DNA is more flexible and can adopt various topologies & secondary structures.

Reduced immunogenicity

Single-stranded DNA is less toxic than double-stranded DNA, enabling safer and more repeatable dosing of genetic medicines.

Our Manufacturing Technology

Fermentation-based single-stranded DNA synthesis

Our manufacturing process enables the scalable production of gram-level quantities of single-stranded DNA while ensuring clonal sequence fidelity.

Conventional single-stranded DNA production methods face significant challenges in scale, sequence length, sequence flexibility and fidelity.

At gxstrands, we overcome these limitations through our advanced fermentation-based technology. This approach enables the production of single-stranded DNA with virtually any sequence length, available in both circular and linear formats, and at gram-scale quantities.

Our patented production process uses specialized DNAzymes to achieve precise linearization. Unwanted residues, including backbone sequence, are effectively removed, leaving only an AG at the 5' end. This ensures high precision and suitability for demanding applications.

Sources

1. Praetorius F, Kick B, Behler KL, Honemann MN, Weuster-Botz D, Dietz H. Biotechnological mass production of DNA origami. Nature. 2017 Dec 6;552(7683):84-87. doi: 10.1038/nature24650. PMID: 29219963.

2. Behler KL, Honemann MN, Silva-Santos AR, Dietz H, Weuster-Botz D. Phage-free production of artificial ssDNA with Escherichia coli. BiotechnolBioeng. 2022 Oct;119(10):2878-2889. doi: 10.1002/bit.28171. Epub 2022 Jul 18. PMID: 35791494.

3. Xie et al., 2024, ”Efficient non-viral immune cell engineering using circular single-stranded DNA-mediated genomic integration”, Motwani et al., 2019, “DNA sensing by the cGAS–STING pathway in health and disease”